Human HLA‐DRα gene: a rare oligonucleotide (GTATA) identifies an upstream sequence required for nuclear protein binding

FEBS Letters

Barbieri, Rafaella¹  Nastruzzi, Claudio¹  Mileo, Anna Maria³  Ferrini, Umberto³  Barrai, Italo⁵  Soria, Marco²  Volinia, Stefano¹  Gambari, Roberto¹  Giacomini, Patrizio⁴  Natali, Pier Giorgio⁴ 

[1] Istituto di Chimica Biologica, Università di Ferrara, FerraraItaly;Farmitalia, Carlo Erba, MilanoItaly;Laboratorio di Biofisica, Istituto Regina Elena, Roma, Italy;Laboratorio di Immunologia, Istituto Regina Elena, Roma, Italy;Istituto di Zoologia, Universitá di Ferrara, Ferrara, Italy

关键词: Oligonucleotide;    DNA-binding factor;    HLA-DRα;    mer;    oligonucleotide;    Mb;    megabase;    bp;    base pairs;    CUS;    conserved upstream DNA sequences;   

DOI  :  10.1016/0014-5793(90)80970-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthetic oligonucleotides containing putative regulatory sequences are currently employed to identify and isolate genes coding for nuclear binding factors. Upstream DNA sequences of eukaryotic genes required for transcriptional activity and tissue specificity can be identified by means of biochemical techniques as well as computer analysis using homology searching. An alternative approach has been recently proposed by our research group. Scanning DNA sequences 1.8 megabases in length from a Genetic Sequence Data Bank, we have identified rare oligonucleotides 5 base pairs (bp) long, which are localized within or close to regulatory segments in mammalian promoters. In this paper we demonstrate that the rare GTATA sequence identifies an upstream region of the HLA-DRα gene which operates in conjunction with the sequence AGAAGTCAG, homologous to a box found in many interferon-inducible genes, in binding nuclear proteins.

【 授权许可】

Unknown