Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme

FEBS Letters

Kindl, Helmut¹  Melchior, Frauke¹ 

[1] University of Marburg, Department of Chemistry, Hans-Meerwein-Strasse, D-3550 Marburg, FRG

关键词: Bacterial expression;    Chalcone synthase;    Stilbene synthase sequence;    Vitis;   

DOI  :  10.1016/0014-5793(90)80961-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into the vectors pKK233-2 and pDS12/RBSII-2, respectively. Expression of the cDNA in Escherichia coli yielded an enzyniatically active dimer exhibiting solely stilbene synthase activity. The protein was characterized by enzyme activity and Western blot analysis.

【 授权许可】

Unknown