Differential sensitivity of bicarbonate‐reversible formate effects on herbicide‐resistant mutants of Synechocystis 6714

FEBS Letters

Vernotte, Claudie¹  Etienne, Anne-Lise¹  Astier, Chantal¹  Peteri, Brigitta¹ 

[1] Department of Physiology and Biophysics, UPR407 Photosynthèse, Bâtiment 24, CNRS, 91198 Gif-sur-Yvette, France

关键词: Photosystem II;    Herbicide;    Cyanobacterial mutant;    D-I protein;    Bicarbonate;    Synechocystis 6714;    Chl;    chlorophyll;    D-1;    a 32 kDa intrinsic protein of the reaction center of PS II;    D-2;    another 32 kDa intrinsic protein of the reaction center of PS II;    DCMU;    3-(3;    4-dichlorophenyl)-l;    l-dimethylurea;    PS II;    photosystem II;    QA;    primary plastoquinone electron acceptor of PS II;    a one-electron acceptor;    QB;    secondary plastoquinone electron acceptor of PS II;    a two-electron acceptor;   

DOI  :  10.1016/0014-5793(90)80943-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Herbicide-resistant mutants of the cyanobacterium Synechocystis 6714, that are altered in specific amino acids in their D-l protein, show differential sensitivity to formate treatment. Measurements on oxygen yield in a sequence of flashes, chlorophyll (Chl) a fluorescence transients and Chl a fluorescence yield decay after a flash reveal that the resistance of cells to formate treatment is in the following (highest to lowest) order: [double mutant] A251V/F211S (Az V) > [single mutant] F211S (Az I) ⋍wild type > [single mutant] S264A (DCMU II-A). Significance of these results in terms of overlapping between the herbicide and bicarbonate binding niches on the D-I protein is discussed.

【 授权许可】

Unknown