FEBS Letters | |
The three‐dimensional structure of porin from Rhodobacter capsulatus at 3 Å resolution | |
Weiss, M.S.2  Schulz, G.E.2  Wacker, T.3  Weite, W.3  Weckesser, J.1  | |
[1] Institut für Biologie II, Mikrobiologie, Schänzlestr. 1, D-7800 Freiburg i.Br., FRG;Institut für Organische Chemie und Biochemie, Albertstr., D-7800 Freiburg i.Br., FRG;Institut für Biophysik und Strahlenbiologie, Albertstr., D-7800 Freiburg i.Br., FRG | |
关键词: Porin; Membrane protein structure; X-ray structure; Rhodobacter capsulatus; | |
DOI : 10.1016/0014-5793(90)80942-C | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The crystal structure of porin from Rhodobacter capsulatus strain 37b4 has been solved at 3.0 Å (1 Å = 0.1 nm) resolution by multiple isomorphous replacement and solvent-flattening. The three pores of the trimer are well denned in the electron density map. Each pore consists of a 16-stranded β-barrel which traverses the membrane as a tube. Near its center the tube is narrowed by chain segments protruding from the inner wall of the barrel that form an eye-let with an irregular cross-section of about 6 Å by 10 Å. The eye-let has an axial length of about 10 Å; it defines the exclusion limit for diffusing particles.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020293600ZK.pdf | 448KB | download |