RGD peptides may only temporarily inhibit cell adhesion to fibronectin

FEBS Letters

Ylänne, Jari¹ 

[1] Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, 00170 Helsinki, Finland

关键词: Cell adhesion;    Fibronectin;    Vitronectin;    Integrin;    Fibronectin peptide;   

DOI  :  10.1016/0014-5793(90)80283-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When human fibroblasts were cultured on fibronectin for 4 h in the presence of 0.5 of the GRGDSP peptide derived from the fibronectin cell-binding site, they adhered and spread normally and organized talin and integrin α₅ and β₁ subunits into focal adhesions. When the adherent cells were quantitated as a function of time, submaximal peptide concentrations were found to delay cell adhesion on fibronectin, but they had no effect on the maximum. When the cells were plated on vitronectin, however, even relatively low peptide concentrations lowered the maximal amount of cells adhering and abolished cell spreading. The results suggest a different mechanism for cell adhesion on fibronectin and vitronectin.

【 授权许可】

Unknown