FEBS Letters | |
Two‐dimensional crystallization of DNA gyrase B subunit on specifically designed lipid monolayers | |
Mioskowski, C.1  Schultz, P.1  Lebeau, L.1  Wang, J.C.2  Oudet, P.3  Regnier, E.1  | |
[1] Laboratoire de Synthèse Bio-organique, CNRS, Unité de Recherche Associée 1386, Faculté de Pharmacie, 74, route du Rhin, 67401 Illkirch, France;Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138, USA;Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, 11, rue Humann, 67085 Strasbourg Cédex, France | |
关键词: DNA gyrase; Novobiocin; Protein-lipid interaction; Two-dimensional crystallization; Electron microscopy: Image processing; | |
DOI : 10.1016/0014-5793(90)80282-N | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The B subunit of DNA gyrase formed two-dimensional crystals when bound to a specifically recognized phospholipid spread into a monolayer at the air/water interface. The especially designed lipids consisted of novobiocin coupled through the 3' or 2″ hydroxyl group and a hydrophilous linker of a given length to dioleoylphosphatidic acid. Two-dimensional crystals of the gyrase B subunit are formed under physiological conditions of pH and ionic strength, with no precipitant added to the solution. Crystal diffraction extended to a 2.7 nm resolution in negative stain, with unit cell parameters a = 6.1 nm, b = 7.6 nm and γ = 64°.
【 授权许可】
Unknown
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