Two‐dimensional crystallization of DNA gyrase B subunit on specifically designed lipid monolayers

FEBS Letters

Mioskowski, C.¹  Schultz, P.¹  Lebeau, L.¹  Wang, J.C.²  Oudet, P.³  Regnier, E.¹ 

[1] Laboratoire de Synthèse Bio-organique, CNRS, Unité de Recherche Associée 1386, Faculté de Pharmacie, 74, route du Rhin, 67401 Illkirch, France;Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138, USA;Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, 11, rue Humann, 67085 Strasbourg Cédex, France

关键词: DNA gyrase;    Novobiocin;    Protein-lipid interaction;    Two-dimensional crystallization;    Electron microscopy: Image processing;   

DOI  :  10.1016/0014-5793(90)80282-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The B subunit of DNA gyrase formed two-dimensional crystals when bound to a specifically recognized phospholipid spread into a monolayer at the air/water interface. The especially designed lipids consisted of novobiocin coupled through the 3' or 2″ hydroxyl group and a hydrophilous linker of a given length to dioleoylphosphatidic acid. Two-dimensional crystals of the gyrase B subunit are formed under physiological conditions of pH and ionic strength, with no precipitant added to the solution. Crystal diffraction extended to a 2.7 nm resolution in negative stain, with unit cell parameters a = 6.1 nm, b = 7.6 nm and γ = 64°.

【 授权许可】

Unknown