NMR studies of the lipid metabolism of T47D human breast cancer spheroids

FEBS Letters

Degani, H.¹  Ronen, S.M.¹  Stier, A.² 

[1] Isotope Department, The Weizmann Institute of Science, Rehovot, 76100 Israel;Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg, D-3400 Göttingen, FRG

关键词: Nuclear magnetic resonance;    T47D;    Breast cancer;    Lipid metabolism;    Phosphomonoester;    Spheroid;    MRI;    magnetic resonance imagining;    MRS;    magnetic resonance spectroscopy;    NMR;    nuclear magnetic resonance;    NTP;    nucleotide triphosphate;    PME;    phosphomonoesters;    PE;    phosphorylethanolamine;    PC;    phosphorylcholine;    Pdy1E;    phosphatidylethanolamine;    Pdy1C;    phosphatidylcholine;   

DOI  :  10.1016/0014-5793(90)81526-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in vivo ³¹P NMR spectrum of T47D human breast cancer cells grown as spheroids shows changes in the phosphomonoester lipid precursors as a function of spheroid size. The ratio of phosphorylethanolamine (PE) to phosphorylcholine (PC) was 1.0±0.3 for 3-day-old, 150 μm spheroids. This ratio increased to 2.4±0.4 for spheroids 7 days and older and which were at least 300 μm in diameter. To investigate the phosphatidylethanolamine to phosphatidylcholine (PdyIE/Pdy1C) ratio in the membranes, chloroform/methanol extracts of spheroids were performed. The ³¹P spectrum of these extracts showed no change with spheroid size, namely the PdyIE/Pdy1C ratio was 0.5±0.06 for spheroids of all ages suggesting that membrane composition is strongly regulated at the precursor level.

【 授权许可】

Unknown