Cyclic nucleotide‐activated channels in the frog olfactory receptor plasma membrane

FEBS Letters

Zhainazarov, A.B.¹  Kolesnikov, S.S.¹  Kosolapov, A.V.¹ 

[1] Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR

关键词: Olfactory receptor cell;    Cyclic nucleotide-activated channel;    Single channel recording;    cAMP;    cyclic adenosine-3′;    5′-monophosphate;    cGMP;    cyclic guanosine-3′;    5′-monophosphate;    8BrcGMP;    cyclic 8-bromoguanosine-3′;    5′-monophosphate;    ATP;    adenosine-5′-triphosphate;    GTP;    guanosine-5′-triphosphate;    EDTA;    ethylenedia-minetetraacetic acid;    EGTA;    ethylene glycol bis(2-aminoethylether)-N;    N;    N′-tetraacetic acid;    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;    DCPA;    3′;    4′-dichlorobenzamil;    OR;    olfactory receptor cell;    PR;    photoreceptor cell;    CN-channels (conductance);    cyclic nucleotide-activated channels (conductance);    low Ca2+;    Mg2+ solution;    solution containing 10−7 M Ca2+ and Mg2+ or less;   

DOI  :  10.1016/0014-5793(90)81515-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Patch clamp technique was used to record cyclic nucleotide-dependent current of the frog olfactory receptor cell plasma membrane. Data obtained indicate that the channels passing this current are permeable to Ca²⁺ or Mg²⁺ and moderately selective for monovalent cations according to the sequence Li⁺, Na⁺, K⁺ > Rb⁺ > Cs⁺ and are effectively blocked by 1-cis-diltiazem and 3′,4′-dichlorobenzamil. The conductance of single cyclic nucleotide-gated channels in solutions with low Ca²⁺ and Mg²⁺ content is about 19 pS. The results demonstrate that cyclic nucleotide-activated channels of olfactory receptor cells are virtually identical to photoreceptor ones.

【 授权许可】

Unknown