Subcellular distribution of α, and β subunit proteins of Ca2+ /calmodulin‐dependent protein kinase II expressed in Chinese hamster ovary cells

FEBS Letters

Ohsako, Shunji¹  Yamauchi, Takashi¹  Sekihara, Shun-ichi¹ 

[1] Department of Neurochemistry, Tokyo Metropolitan Institute for Neurosciences, 2-6 Musashidai, Fuchu-City, Tokyo 183, Japan

关键词: Calcium ion;    Calmodulin;    Protein kinase;    Protein phosphorylation;    Subcellular distribution;    Expression of cDNAs;   

DOI  :  10.1016/0014-5793(90)81505-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When two cDNAs respectively encoding the entire coding regions of α and β subunits of Ca²⁺ /calmodulin-dependent protein kinase II (CaM kinase II) were introduced into Chinese hamster ovary cells, the expressed α and β subunits were differently associated with subcellular structure. Although α subunit was loosely associated with subcellular structure, about 80% of CaM kinase II acitivity of α subunit was found in soluble fraction. More than 50% of the β subunit bound to the membrane, and the remainder was soluble but was loosely associated with subcellular structure. The relative rate of phosphorylation for substrate proteins of the β subunit bound to membrane was significantly different from that of the soluble form.

【 授权许可】

Unknown