Two‐dimensional crystallization of reaction centers from Chloroflexus aurantiacus

FEBS Letters

Demin, V.V.¹  Zargarov, A.A.¹  Barnakov, A.N.¹  Kuzin, A.P.¹  Abdulaev, N.G.¹  Zolotarev, A.S.¹ 

[1] Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871 GSP Moscow V-437, USSR

关键词: Reaction center;    Two-dimensional crystal;    Electron microscopy;    Image processing;    LDAO;    lauryldimethylamine N-oxide;    RC;    reaction center;   

DOI  :  10.1016/0014-5793(90)80900-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two-dimensional crystals of photosynthetic reaction centers from Chloroflexus aurantiacus were obtained from protein-lipid-detergent micelles by detergent dialysis. The size of crystals was up to 2 μm. Some of them were multilayered crystals. However, other crystal forms were also observed. Preliminary image processing analysis showed that crystals of one crystal form referred to two-sided plane group p2 and had the following unit cell parameters: a = 17,6 nm, b = 18,0 nm, γ = 84°. The contour map of the crystal stain-excluding region was calculated by the Fourier-filtering procedure at about 2 nm resolution.

【 授权许可】

Unknown