Translational regulation of plastid gene expression in Euglena gracilis

FEBS Letters

Parthier, Benno¹  Reinbothe, Steffen¹ 

[1] Institute for Plant Biochemistry, Academy of Sciences of GDR, Weinberg 3, Halle 4050, GDR

关键词: Plastid gene expression;    Elongation of translation;    Glutamyl-tRNA limitation;    Glutamate codon frequency in protein genes;    Euglena gracilis;   

DOI  :  10.1016/0014-5793(90)80870-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Translation of plastid messenger RNAs depends on aminoacyl-tRNAs formed by charging plastid-encoded tRNAs with cognate amino acids. The enzymes involved, chloroplast aminoacyl-tRNA synthetases, are encoded in the nucleus. Both the tRNAs and the aminoacyl-tRNA synthetases are stimulated in synthesis if dark-grown cells are exposed to light. However, their accumulation during light-induced chloroplast development in Euglena gracilis starts with an appreciable lag-phase. During this period the availability of charged tRNAs probably limits protein synthesis. Due to the contemporary need of glutamyl-tRNA^Glu _GAA in chlorophyll synthesis this particular tRNA is very likely depleted. Based on an analysis of glutamate codon frequency in known plastid genes, the effect of a glutamyl-tRNA^Glu _GAA limitation on the translation of plastid messages is discussed.

【 授权许可】

Unknown