cDNA cloning and sequencing of component C9 of proteasomes from rat hepatoma cells

FEBS Letters

Onikura, Aya³  Fujiwara, Tsutomu⁴  Tanaka, Keiji²  Tokunaga, Fuminori¹  Tamura, Tomohiro²  Iwanaga, Sadaaki³  Kumatori, Atsushi²  Ichihara, Akira² 

[1] Department of Molecular Biology, Graduate School of Medical Science, Kyushu University 33, Fukuoka 812, Japan;Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan;Department of Biology, Faculty of Science, Kyushu University 33, Fukuoka 812, Japan;Otsuka Pharmaceutical Company Ltd., Tokushima 771-01 Japan

关键词: cDNA cloning;    Multicatalytic proteinase;    Proteasome;    Subunit C9;    SDS-PAGE;    sodium dodecyi sulfate-polyacrylamide gel electrophoresis;    HPLC;    high-performance liquid chromatography;    bp;    base pairs;   

DOI  :  10.1016/0014-5793(90)80267-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of component C9 of rat proteasomes (multicatalytic proteinase complexes) has been determined from a recombinant cDNA clone isolated by screening a Reuber H4TG hepatoma cell cDNA library using synthetic oligodeoxynucleotide probes corresponding to partial amino acid sequences of the protein. The predicted sequence ofC9 consists of 261 amino acid residues with a calculated molecular weight of 29496. The C9 component is a novel protein, differing from known proteins, but its primary structure resembles those of other proteasome components, including C2, C3 and C5, although its similarity to C5 is relatively low, suggesting that proteasomes consist of a family of proteins that have evolved from a common ancestor.

【 授权许可】

Unknown