Identification of 17–18 kDa zona pellucida binding proteins from boar spermatozoa

FEBS Letters

Pěknicová, J.³  Pavlík, M.¹  Moos, J.³  Sumeva-Nakova, Ts.N.² 

[1] Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Flemingovo nám. 2, 166 37 Prague 6, Czechoslovakia;Institute of Biology and Immunology of Reproduction and Development of Organisms, Bulgaria Academy of Sciences, Lenin Ave 73, Sofia 1113, Bulgaria;Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Videňská 1083, 142 20 Prague 4, Czechoslovakia

关键词: Zona pellucida binding protein;    Boar sperm cell;    Acrosin;    Fucose;    Amino acid sequence;    BSA;    bovine serum albumin;    HPLC;    highperformance liquid chromatography;    PTH;    phenylthiohydantoin;    SDS;    sodium dodecyi sulphate;    UEA;    Ulex europaeus agglutinin;    ZP;    zona pellucida;   

DOI  :  10.1016/0014-5793(90)80258-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Zona pellucida (ZP) binding proteins from boar spermatozoa were compared with antigens recognized by ACR.2 and ACR.3 monoclonal antibodies. The ZP binding proteins of 55, 53,45 and 38 kDa are identical with various forms of boar acrosin immunologically detected by ACR.2 antibody. The ZP binding proteins of 17 and 18 kDa are recognized by ACR.3 antibody. The N-tenninal amino acid sequence of the 17 kDa protein revealed that it is not derived from an acrosin molecule.

【 授权许可】

Unknown