Microtubule translocation caused by three subspecies of inner‐arm dynein from Chlamydomonas flagella

FEBS Letters

Kagami, Osamu¹  Takada, Saeko¹  Kamiya, Ritsu¹ 

[1] Department of Molecular Biology, School of Science, Nagoya University, Nagoya 464-01, Japan

关键词: Dynein;    Microtubule;    Motility;    Translocation;   

DOI  :  10.1016/0014-5793(90)80243-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To help understand the function of inner-arm dynein in nagellar motility, dynein samples from an outer arm-missing mutant of Chlamydomonas (odal) were examined for the ability to translocate microtubules in vitro. High-salt extract ofaxonemes containing inner-arm dynein was separated by ion-exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5 , which were comparable to the microtubule sliding rate in disintegrating oda axonemes.

【 授权许可】

Unknown