Rate enhancement of the electron transfer of the adrenodoxin‐adrenodoxin reductase system by inorganic and nucleotide phosphates

FEBS Letters

Nonaka, Yasuki¹  Yamano, Toshio²  Miura, Retsu² 

[1] Departments of Biochemistry, and Molecular and Physiological Chemistry, Osaka University Medical School, 4-3-57 Nakanoshima, Kita-ku, Osaka 530, Japan;Laboratory of Chemistry, Kansai Medical University, 18-19 Uyamahigashi, Hirakata 573 Japan

关键词: Adrenodoxin;    Adrenodoxin reductase;    Adrenocortical mitochondria;    Electron transfer;    Pyrophosphate;    ATP;   

DOI  :  10.1016/0014-5793(90)80784-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphate and pyrophosphate increased the rate of reduction of adrenodoxin by NADPH-adrenodoxin reductase and NADPH, pyrophosphate being one order more effective than the former. However, the cytochrome c reduction by the electron transport system was inhibited in the presence of inorganic (pyro)ptiosphate. On the other hand, ADP and ATP enhanced the rates of reduction of both adrenodoxin and cytochrome c through adrenodoxin by the electron transport system. GTP also enhanced the rate of reduction of cytochrome c by this system, whereas AMP showed no appreciable enhancement. These inorganic and nucleotide phosphates did not affect the rate of ferricyanide reduction by the reductase.

【 授权许可】

Unknown