Rats with physically disconnected hypothalamo‐pituitary tracts no longer contain vasopressin‐oxytocin gene transcripts in the posterior pituitary lobe

FEBS Letters

Mohr, Evita¹  Zhou, An¹  Thorn, Niels A.¹  Richter, Dietmar¹ 

[1] Institut für Zellbiochemie und klinische Neurobiologie, UKE, Martinistr. 52, 2000 Hamburg 20, FRG

关键词: Vasopressin gene;    Oxytocin gene;    Posterior pituitary;    Electrically lesioned animal;    Pituicyte;   

DOI  :  10.1016/0014-5793(90)81407-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In rats, vasopressin- and oxytocin-encoding mRNAs are present in the posterior but absent in the anterior lobe of the pituitary gland. RNase protection experiments indicate that in the posterior pituitary and hypothalamus identical transcriptional start points are used. Furthermore, the two transcripts from posterior pituitary and hypothalamus show identical nucleotide sequences. Animals operated by paired electrical lesions in such a way that connections between the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of the hypothalamus and the posterior pituitary lobeare destroyed continue to express the vasopressin and oxytocin gene in the hypothalamus but not in the posterior pituitary. Operated animals subjected to chronic intermittent salt loading for 6 days similarly contain vasopressin and oxytocin encoding transcripts in the hypothalamus but not in the posterior pituitary.

【 授权许可】

Unknown