Application of 13C(ω1)‐half‐filtered [1H,1H]‐NOESY for studies of a complex formed between DNA and a 13C‐labeled minor‐groove‐binding drug

FEBS Letters

Leupin, W.¹  Otting, G.¹  Wüthrich, K.¹  Amacker, H.¹ 

[1] Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg, CH-8093 Zürich, Switzerland

关键词: Nuclear magnetic resonance;    Drug/oligodeoxyribonucleotide complex;    [1H;    1H]-NOESY with 13C(ω1)-half-filter;    Intermolecular nuclear Overhauser efrect;   

DOI  :  10.1016/0014-5793(90)81402-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complex formed between the anticancer drug 4-[p-[p-(4-quinolylamino)benzamido]anilino]pyridine (SN 6999) and the decadeoxyribonucleoside nonaphosphate d-(GCATTAATGC)₂ was investigated using two-dimensional nuclear Overhauser enhancement spectroscopy (NOESY) with a ¹³C(ω₁)-half-filter. The two quaternary methyl groups in SN 6999 had been labeled with ¹³C for these experiments. The simplified subspectra of [¹H,¹H]-NOESY obtained with this procedure greatly facilitate the identification and assignment of intermolecular NOEs. Quite generally, the combined use of isotope labeling and heteronuclear filters in [¹H,¹H]-NOESY provides an improved experimental basis for structural studies of drug/ DNA complexes.

【 授权许可】

Unknown