Oriented incorporation of bacteriorhodopsin into the lipid shell of phospholipid‐coated polymer particles

FEBS Letters

Engelhardt, Harald¹  Aurich, Harald¹  Rothe, Ulrich¹  Oesterhelt, Dieter¹ 

[1] Institut für Biochemie der Martin-Luther-Universität, DDR-4010 Halle/S., GDR

关键词: Phospholipid-coated bead;    Membrane model;    Bacteriorhodopsin;    BR;    bacteriorhodopsin;    PE;    phosphatidylethanolamine;    EDC;    1-ethyl;    3-(3-dimethylaminopropyl);    carbodiimide;    PM;    purple membrane;   

DOI  :  10.1016/0014-5793(90)81401-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phospholipid monolayers covalently fixed to spherical polymer carriers spontaneously form a stable bilayer-like structure by adsorption of further added phospholipids. Bacteriorhodopsin could be incorporated into the lipid bilayer growing on these particles. From the direction of proton pumping as well as from freeze-fracture electron micrographs and papain digestion, experimental evidence was obtained that the preferred orientation of bacteriorhodopsin within the lipid coat is ‘inside-out’ (more than 90%). A ‘right-side-out’ incorporation of bacteriorhodopsin could be demonstrated, if the negative charge density of the carrier surface was lowered by chemical modification.

【 授权许可】

Unknown