A rev/β‐galactosidase fusion protein binds in vitro transcripts spanning the rev‐responsive element of human immunodeficiency virus type 1 (HIV‐1)

FEBS Letters

Bröker, Michael²  Fleckenstein, Bernhard¹  Voll, Reinhard¹  Aepinus, Christian¹ 

[1] Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen FRG;Behringwerke AG, D-3550 Marburg, FRG

关键词: Rev-protein;    Human immunodeficiency virus type 1;    Sequence-specific RNA-binding;    Rev responsive element;    Immunoprecipitation;    Northwestern blot;   

DOI  :  10.1016/0014-5793(90)81377-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rev protein of human immunodeficiency virus type 1 (HIV-1), a phosphoprotein of 20 kDa apparent molecular mass, is essential to target the mRNA for virion polypeptides into the cytoplasm. This effect is mediated by a specific RNA stretch (rev-responsive element = RRE) localized within a 3′-terminal segment of the mRNA encoding virion proteins. We present evidence that rev expressed as a β-galactosidase fusion protein in E. coli forms a complex with in vitro transcripts containing the RRE; it can be precipitated by monoclonal antibodies with rev or β-galactosidasespecificity. In addition, specific binding of rev protein to RNA could be demonstrated by Northwestern blotting.

【 授权许可】

Unknown