期刊论文详细信息
FEBS Letters
A rev/β‐galactosidase fusion protein binds in vitro transcripts spanning the rev‐responsive element of human immunodeficiency virus type 1 (HIV‐1)
Bröker, Michael2  Fleckenstein, Bernhard1  Voll, Reinhard1  Aepinus, Christian1 
[1] Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, D-8520 Erlangen FRG;Behringwerke AG, D-3550 Marburg, FRG
关键词: Rev-protein;    Human immunodeficiency virus type 1;    Sequence-specific RNA-binding;    Rev responsive element;    Immunoprecipitation;    Northwestern blot;   
DOI  :  10.1016/0014-5793(90)81377-Z
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The rev protein of human immunodeficiency virus type 1 (HIV-1), a phosphoprotein of 20 kDa apparent molecular mass, is essential to target the mRNA for virion polypeptides into the cytoplasm. This effect is mediated by a specific RNA stretch (rev-responsive element = RRE) localized within a 3′-terminal segment of the mRNA encoding virion proteins. We present evidence that rev expressed as a β-galactosidase fusion protein in E. coli forms a complex with in vitro transcripts containing the RRE; it can be precipitated by monoclonal antibodies with rev or β-galactosidasespecificity. In addition, specific binding of rev protein to RNA could be demonstrated by Northwestern blotting.

【 授权许可】

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