Demonstration of two isoforms of subunit VIIa of cytochrome c oxidase from human skeletal muscle

FEBS Letters

Van den Bogert, Coby¹  Tager, Joseph M.¹  Van Bun, Stefaan²  Muijsers, Anton O.¹  Van Beeumen, Jozef J.²  Van Kuilenburg, André B.P.¹ 

[1] E.C. Slater Institute of Biochemical Research, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands;Laboratory of Microbiology and Microbal Genetics, University of Gent, Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Human cytochrome c oxidase isoenzyme;    Mitochondrial myopathy;   

DOI  :  10.1016/0014-5793(90)81376-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two different isoforms of subunit VIIa have been found in cytochrome c oxidase isolated from human skeletal muscle. The first 22 residues of the N-terminal amino acid sequences showed 5 differences. Our results provide the first conclusive evidence for the existence of cytochrome c oxida isoenzymes in man. Since the two cytochrome c oxidase isoforms were both present in skeletal muscle tissue, though not necessarily in the same cell type, this suggests that human cytochrome c oxidase isoforms are not strictly tissue-specific. These findings may have important implications for the elucidation of genetic diseases in man in which a deficiency of cytochrome c oxidase is restricted to certain tissues.

【 授权许可】

Unknown