Inactivation of Ca2+‐, Na+K+‐, and H+K+‐ATPases with a carbodiimide derivative of ATP

FEBS Letters

Murphy, Alexander J.¹ 

[1] Department of Biochemistry, University of the Pacific, 2155 Webster St., San Francisco, CA 94115, USA

关键词: ATPase;    Affinity label;    Carbodiimide;    ATP derivative;    SR sarcoplasmic reticulum;    Mops 3-(N-morpholino)propanesulionic acid;    TEOA;    triethanolamine;    EGTA;    ethylene glycol bis(2-aminoethyl ether) N;    N;    N';    N'-tetraacetic acid;    AMP-PNP;    adenyl-5'-ylimidodiphosphate;    AMP-PCP;    adenyl-5'-ylmethylenediphosphonate;    TNP-ADP;    2';    3'-(2;    4;    6-trinitro-cyclohexadienylidene) adenosine 5'-diphosphate;    EDC;    1-ethyl-3-(3-diiriethylaminopropyl)-carbodiimide hydrochloride;    EP;    phosphorylated enzyme intermediate;    ATP-EDC;    αmma;    Padduct of ATP and EDC;    PK;    pyruvate kinase;    LDH;    lactate dehydrogenase;    PEP;    phosphoenol pyruvate;   

DOI  :  10.1016/0014-5793(90)80733-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The αmma;P adduct of ATP with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (ATP-EDC) was synthesized and incubated with the Ca-ATPase of sarcoplasmic reticulum with the result that time-dependent complete loss of the enzyme's activity occurred. The inactivation required calcium and magnesium while ATP had a protective effect. ATP-EDC incubation with the NaK-ATPase and HK-ATPase produced partial (> 50%) inactivation, but had no effect on myosin Sl, pyruvate kinase and hexokinase, suggesting that this ATP analog is a specific inactivator of the so-called ’quo;P-type’ ATPases.

【 授权许可】

Unknown