Immunological determination of the oligomeric form of mitochondrial creatine kinase in situ

FEBS Letters

Vial, Christian¹  Quemeneur, Eric¹  Eichenberger, Denise¹ 

[1] LBTM-CNRS (UMR 9), Université Claude Bernard Lyon I, 63 bd. du 11 novembre 1918, F-69622 Villeurbanne Cedex, France

关键词: ELISA analysis;    Immunoinhibition;    Mitochondrial creatine kinase;    Octameric structure;    (Rabbit heart mitochondria);    CKm;    mitochondrial creatine kinase;    CKm1;    dimeric structure;    CKm2;    octameric structure;   

DOI  :  10.1016/0014-5793(90)80209-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Whereas factors governing the interconversion of the two oligomeric forms of mitochondrial creatine kinase are relatively well known, few informations are yet available on the actual form in situ. Antibodies against purified pig and rabbit heart mitochondrial creatine kinase were obtained. The former exhibits a marked specificity for the dimer while the second reacts with both dimer and octamer. They allowed to demonstrate that no dimer can be detected in mitochondria and that CKm occurs naturally exclusively as an octamer. We present arguments that the larger part, if not the totality, of the octamer is membrane-bound rather than soluble in the intennembrane space. However, these findings do not refute the previously proposed models for the regulation of CKm activity in the mitochondrion but urge to envisage a more complex one.

【 授权许可】

Unknown