Identification of a group of proteins that are strongly up‐regulated in total epidermal keratinocytes from psoriatic skin

FEBS Letters

Celis, Ariana²  Ratz, Gitte²  Basse, Bodil²  Celis, Julio E.²  Kiil, Jette¹  Lauridsen, Jette B.²  Cruger, Dorthe² 

[1] Department of Plastic Surgery, Aarhus University Hospital, DK-8000 Aarhus C., Denmark;Institute of Medical Biochemistry and Bioregulation Research Centre, Aarhus University,DK-8000 Aarhus C., Denmark

关键词: Skin disease;    Psoriasis;    Protein sythesis;    Marker;    Two-dimensional gel protein database;    2D;    two dimensional;    IEF;    isoelectric focusing;    SSP;    sample spot number;    IL-6;    interleukin-6;    DMEM;    Dulbecco's modified Eagle's medium;   

DOI  :  10.1016/0014-5793(90)80179-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Analysis using two-dimensional (2D) gel electrophoresis of the [³⁵S]-methionine-labelled proteins synthesized by non-cultured total epidermal keratinocytes obtained from normal and psoriatic skin revealed 6 proteins that are strongly up-regulated (5 times or more) in psoriatic skin. These proteins are synthesized at albeit lower levels by keratinocytes from normal and normal-appearing (uninvolved) skin of psoriatic patients, and correspond to isoelectric focusing sample spot numbers 4311 (40.3 kDa), 4003 (12.4 kDa), 5008 (11.9 kDa), 3012 (11.6 kDa), 6016 (11.6 kDa) and 1015 (10;1 kDa) in the normal keratinocyte 2D gel protein database [Celis et al, (1990) Electrophoresis, in press]. These proteins are also detected in the labelling medium indicating that they are at least in part secreted. Given their striking regulatory behavior, these proteins may play a role in the pathogenesis of psoriasis.

【 授权许可】

Unknown