A new superfamily of putative NTP‐binding domains encoded by genomes of small DNA and RNA viruses

FEBS Letters

Gorbalenya, Alexander E.³  Wolf, Yuri I.²  Koonin, Eugene V.¹ 

[1]Institute of Microbiology, USSR Academy of Sciences, Moscow, USSR

[2]Institute of Bioorganic Chemistry, USSR Academy of Sciences, Novosibirsk, USSR

[3]Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region, USSR

关键词: RNA virus;    DNA virus;    NTP-binding sequence pattern;    Helicase;    Virus evolution;    PV1;    poliovirus type 1;    VB3;    Coxsackie virus type B3;    HRV2;    14;    89;    human rhinoviruses;    respective types;    EMCV;    encephalomyocarditis virus;    TMEV;    Theiler murine encephalomyelitis virus;    FMDV;    foot-and-mouth disease virus type A10;    HAV;    hepatitis A virus;    CPMV;    cowpea mosaic virus;    TBRV;    tomato black ring virus;    AAV;    adeno-associated virus;    MVM;    minute virus of mice;    BPV;    bovine parvovirus;    ADV;    aleutian disease virus;    MDV;    mosquito densonucleosis virus;    WDV;    wheat dwarf virus;    MSV;    maize streak virus;    CSMV;    cassava striate mosaic virus;    TGMV;    tomato golden mosaic virus;    CLV;    cassava latent virus;    BCTV;    beet curly top virus;    BKV;    BK virus;    PyV;    human polyoma virus;    BFDV;    budgerigar fledgling disease virus;    HPV 1a;    6b;    18;    human papilloma viruses;    respective types;    BPV1;    bovine papilloma virus type 1;   

DOI  :  10.1016/0014-5793(90)80175-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Statistically significant similarity was revealed between amino acid sequences of NTP-binding pattern-containing domains which are among the most conserved protein segments in dissimilar groups of ss and dsDNA viruses (papova-, parvo-, geminiviruses and P4 bacteriophage), and RNA viruses (picorna-, como- and nepoviruses) with small genomes. Within the aligned domains of 100–120 amino acid residues, three highly conserved sequence segments have been identified, i.e. ‘A’ and ‘B’ motifs of the NTP-binding pattern, and a third, C-terminal motif ‘C’, not described previously. The sequence of the ‘B’ motif in the proteins of the new superfamily is unusually variable, with substitutions, in some of the members, of the Asp residue conserved in other NTP-binding proteins. The ‘C’ motif is characterized by an invariant Asn residue preceded by a stretch of hydrophobic residues. As the new superfamily included a well studied DNA and RNA helicase, T antigen of SV40, helicase function could be tentatively assigned also to the other related viral putative NTP-binding proteins. On the other hand, the possibility of different and/or multiple functions for some of these proteins is discussed.

【 授权许可】

Unknown