Two novel brain proteins, CaBP33 and CaBP37, are calcium‐dependent phospholipid‐ and membrane‐binding proteins

FEBS Letters

Pula, Grazia¹  Giambanco, Ileana¹  Bianchi, Roberta¹  Donato, Rosario¹ 

[1] Section of Anatomy, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, 06100 Perugia, Italy

关键词: CaBP33/CaBP37;    Calcium;    Phospholipid;    Membrane;    Binding;    Annexin;    (Brain);   

DOI  :  10.1016/0014-5793(90)80157-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two acidic Ca²⁺ -binding proteins (CaBP33 and CaBP37) purified from bovine brain have been characterized in terms of immunological properties, heat-sensitivity, electrophoretic mobility, and Ca²⁺-dependent binding to negatively charged phospholipids and to brain membranes. They were induced to bind to membranes by homogenization of brain tissue in the presence of CaCl₂. The membrane-bound CaBP33/CaBP37 mixture resisted extraction with detergents and was solubilized with high concentrations of EGTA/KC1. However, apparent Ca²⁺-independent binding of the two proteins to membranes seemed to occur as well. This latter fraction of membrane-bound CaBP33 and CaBP37 could be solubilized with Triton X-100, indicating that brain membranes normally contain the two proteins as intrinsic components.

【 授权许可】

Unknown