Complete purification of two identical Na+ ‐pump inhibitors isolated from bovine hypothalamus and hypophysis

FEBS Letters

Ricote, Mercedes¹  Illescas, Manuel¹  G-Robles, Rafael¹  Mendez, Enrique¹  Sancho, Jose¹ 

[1] Endocrinology Service, Hospital Ramon y Cajal, Carr. Colmenar Km.9, Madrid 28034, Spain

关键词: Na+;    K+-ATPase;    Sodium pump;    Ouabain;    BSA;    bovine serum albumin;    FFA;    free fatty acid;    HHIF;    hypothalamic;    hypophysary;    inhibitory factor;    HPLC;    high-performance liquid chromatography;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(90)80610-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have completely purified, in parallel, a low molecular weight, non-peptidic, non-lipidic, Na⁺,K⁺-ATPase inhibitory factor from bovine hypothalamic and pituitary tissues. In the final purification step we obtain, from both tissues, a single, homogeneous peak, with a maximal absorbance at 247 nm. This factor, at physiological concentrations of potassium (5–25 mM), inhibits in a dose-response manner Na⁺,K⁺-ATPase and displaces ouabain from its receptor at the enzyme structure. The factor isolated from both tissues is identical, being the specific activity per weight of tissue higher in hypophysis. No factor was found in cerebral cortex, used as tissue control.

【 授权许可】

Unknown