Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides

FEBS Letters

Levitzki, Alexander¹  Engelberg, David¹  Poradosu, Enrique¹  Simchen, Giora² 

[1] Departments of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel;Departments of Genetics, The Institute of Life Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: Adenylyl cyclase;    Schizosaccharomyces pombe;    Yeast;   

DOI  :  10.1016/0014-5793(90)80605-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is localized to the plasma membrane of the cell. The enzyme utilizes Mn²⁺/ATP as substrate and free Mn²⁺ ions as an effector. Unlike the baker yeast Saccharomyces cerevisiae, S. pombe adenylyl cyclase does not utilize Mg²⁺/ATP as substrate and the activity is not stimulated by guanyl nucleotides. The optimal pH for the S. pombe adenylyl cyclase activity is 6.0. The activity dependence on ATP is cooperative with a Hill coefficient of 1.68 ± 0.14.

【 授权许可】

Unknown