Monoclonal antibodies against the human lymphocyte differentiation antigen CD 76 bind to gangliosides

FEBS Letters

Kniep, Bernhard¹  Schwartz-Albiez, Reinhard³  Dörken, Bernd²  Vilella, R.⁴  Mühlradt, Peter F.¹  Moldenhauer, Gerhard³ 

[1] GBF-Institute for Biotechnological Research, D-3300 Braunschweig, FRG;Department of Internal Medicine, University of Heidelberg, D-6900 HeidelbergFRG;Institute of Immunology and Genetics, German Cancer Research Center, D-6900 Heidelberg, FRG;Servei d'Immmunologia, Hosp. Clinic i Provicial, Barcelona 08036, Spain

关键词: 2;    6-Sialosyllactoneotetraosylceramide;    Sialylation;    CD 76;    B lymphocyte;    Immunostaining;    Carbohydrate antigen;   

DOI  :  10.1016/0014-5793(90)80588-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two monoclonal antibodies, HD 66 and CRIS-4, by which the new CD 76 B-cell-associated cluster was defined, bound to several gangliosides (sialic acid containing glycolipids) of different polarity. One of the gangliosides recognized by HD 66 could be identified as NeuAcα2-6Galβl-4GlcNAcβl-3Galβl-4Glc-βl-l'Cer. This antigen was enzymatically synthesized. Sialidase treatment of the ganglioside antigens abolished binding of HD 66 and CRIS-4.

【 授权许可】

Unknown