De novo protein synthesis is essential to human interferon γ gene expression by the stimulation with polyI:polyC

FEBS Letters

Sasakawa, Shigeru¹  Tamura-Nishimura, Motoko¹ 

[1]The Japanese Red Cross, Central Blood Center, Tokyo, Japan

关键词: Interferon γ;    Poly Lpoly C;    Gene expression;    Cycloheximide;    12-O-Tetradecanoylphorbol 13-acetate;    IFN;    interferon;    NNA cells;    nylon-nonadherent cells;    pkC;    protein kinase C;    PBML;    peripheral blood mononuclear lymphocyte;    cAMP;    adenosine 3';    5'-cyclic monophosphate;    CHX;    cycloheximide;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/0014-5793(90)80587-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transcription of human interferon (IFN) γ gene is induced in human peripheral lymphocyte nylon-nonadherent cells (NNA cells) by double strand RNA poly I:poly C [(1985) J. Interferon Res. 5, 77-84]. In this report, the necessity of de novo protein synthesis in an early stage of IFN γ gene expression is described. For induction of IFN γ gene expression, only initial 4 h treatment of poly I:poly C to NNA cells is sufficient. Addition of inhibitor of protein synthesis, cycloheximide (CHX), at an early stage of induction periods (0–4 h) inhibits the IFN γ induction by poly I:poly C. Cell free translation assay using RNAs isolated from NNA cells which are induced by poly I:poly C in the presence of CHX reveals that in these RNAs, IFN γ mRNA does not exist. These results demonstrate that CHX inhibits de novo synthesis of a certain protein (or proteins) and for lack of the protein(s), IFN γ mRNA cannot be transcribed. The evidence is also described in this report which suggests that the essential protein(s) might be that (those) involved in protein kinase C (pkC) activation.

【 授权许可】

Unknown