Stimulatory effect of ammonium sulfate at high concentrations on the aminoacylation of tRNA and tRNA‐like molecules

FEBS Letters

Kern, Daniel¹  Giege, Richard¹  Florentz, Catherine¹ 

[1] Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15 rue René Descartes, 67084 Strasbourg, France

关键词: Valyl-tRNA synthetase;    tRNAVal;    tRNA-like;    Ammonium sulfate;    Aminoacylation;   

DOI  :  10.1016/0014-5793(90)80585-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of various salts on the aminoacylation of tRNA^Val and the tRNA-like structure from turnip yellow mosaic virus RNA by yeast valyl-tRNA synthetase has been studied. As expected, increasing the concentration of salts inhibits the enzymatic reaction. However, in the presence of high concentration of ammonium sulfate, and only this salt, the inhibitory effect is suppressed. Under such conditions, the aminoacylation becomes comparable to that measured in the absence of salt. It was shown that ammonium sulfate affects both the catalytic rate of the reaction and the affinity between valyl-tRNA synthetase and the RNAs. Because the affinity between the partners in the complex is increased when the concentration of the salt is high, it is suggested that hydrophobic effects are involved in tRNA/synthetase interactions.

【 授权许可】

Unknown