Alternative splicing gives rise to two forms of the p68 Ca2+ ‐binding protein

FEBS Letters

Moss, Stephen E.¹  Crumpton, Michael J.¹ 

[1] Cell Surface Biochemistry Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, England

关键词: p68;    Ca2+-binding protein;    Lipocortin;    Calpactin;    Alternative splicing;   

DOI  :  10.1016/0014-5793(90)80576-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The p68 Ca²⁺ and phospholipid binding protein of the lipocortin/calpactin family appears to exist as two forms. These may be resolved into a closely-spaced polypeptide doublet by SDS-PAGE. The cloning and sequencing of p68 revealed an apparent 18 nucleotide alternative splice sequence, which could account for this observation. We show here that an antiserum directed against a synthetic peptide corresponding to the region containing the splice sequence, recognises only the upper band of the p68 doublet by both immunoprecipitation and Western blotting., These results are consistent with alternative splicing being responsible for the generation of the two forms of p68.

【 授权许可】

Unknown