Simultaneous recordings of glucose dependent electrical activity and ATP‐regulated K+‐currents in isolated mouse pancreatic β‐cells

FEBS Letters

Rorsman, Patrik¹  Smith, Paul A.¹  Ashcroft, Frances M.¹ 

[1] University Laboratory of Physiology, Oxford University, Parks Road, Oxford OX1 3PT, England

关键词: Insulin secretion;    ATP-regulated K+-channels;    Glucose;    Nystatin;   

DOI  :  10.1016/0014-5793(90)80667-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Membrane potential and membrane currents were recorded from single mouse pancreatic β-cells using the perforated patch whole-cell recording technique at 30 °C. Single β-cells maintained in primary tissue culture exhibited glucose-dependent electrical activity similar to that reported for freshly isolated intact islets. The resting input conductance (5.1±0.9 nS) was determined by ATP-regulated K⁺ (K_ATP) channels as it was blocked by 1 mM tolbutamide. 8 mM glucose decreased the input conductance by 80%. The input conductance at −70 mV was of a similar value during the plateau phase and during the silent phase of electrical activity in 8 mM glucose. This suggests that oscillations of K_ATP channel activity do not underlie the slow waves.

【 授权许可】

Unknown