Transport of malate and chloride into barley mesophyll vacuoles Different carriers are involved

FEBS Letters

Vogt, Esther¹  Martinoia, Enrico¹  Amrhein, Nikolaus¹ 

[1] Institut für Pflanzenwissenschaften, Biochemie und Physiologie der Pflanzen, Sonneggstrasse 5, ETH-Z, CH-8092 Zürich, Switzerland

关键词: Vacuole;    Transport;    Malate;    Chloride;   

DOI  :  10.1016/0014-5793(90)80648-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transport of malate and chloride across the tonoplast of barley mesophyll vacuoles has been compared. Whereas malate inhibited chloride uptake, 1,2,3-benzenetricarboxylic acid, a potent inhibitor of malate uptake which is assumed not to cross the tonoplast, was ineffective. In contrast to chloride uptake, malate uptake was inactivated by pyridoxal phosphate and phenylglyoxal. The malate transport system could be protected against pyridoxal phosphate when a substrate such as 1,2,3-benzenetricarboxylic acid was present in the preincubation medium. It is concluded that the transfer of malate and chloride across the tonoplast is mediated by different systems.

【 授权许可】

Unknown