Inactivation of cGMP‐dependent conductance of rod outer segment plasma membrane induced bij cGMP

FEBS Letters

Zhainazarov, A.B.¹  Kolesnikov, S.S.¹ 

[1] Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR

关键词: cGMP-dependent channel;    Rod outer segment;    Channel operation kinetics;    cGMP;    cyclic guanosine-3';    5'-monophosphate;    SBrcGMP;    cyclic 8-bromo-guanosine-3';    5'-monophosphate;    EDTA;    ethylenediamine tetraacetic acid;    EGTA;    ethylene glycol bis(2-aminoethylether)-N;    N;    N'-tetraacetic acid;    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;    ROS;    rod outer segment;    RIS;    rod inner segment;   

DOI  :  10.1016/0014-5793(90)80089-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP concentration results from a decrease in channel opening probability. Thus, cGMP in high concentrations inactivates cGMP-dependent conductance.

【 授权许可】

Unknown