Inositol trisphosphate‐mediated Ca2+ release in beet mu;somes is inhibited by heparin

FEBS Letters

Brosnan, James M.¹  Sanders, Dale¹ 

[1] Biology Department, University of York, York Y01 5DD, England

关键词: Tonoplast;    Calcium efflux;    Inositol 1;    4;    5-trisphosphate;    Heparin;    (Beta vulgaris);    BTP;    1;    3-bisltris(hydroxymethyl)methylamino]-propane;    InsP3;    inositol 1;    4;    5-trisphosphate;    InsP2;    inositol 1;    4-bisphosphate;    InsP4;    inositol 1;    3;    4;    5-tetrakisphosphate;    TMB-8;    8-(N;    N-diethylamino)-octyl 3;    4;    5-trimethoxybenzoate-HCl;    FCCP;    carbonylcyanide p-trifluoromethoxyphenylhydrazone;   

DOI  :  10.1016/0014-5793(90)80068-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Microsomes from the storage root of red beet exhibit ATP-dependent, protonophore-sensitive Ca²⁺ accumulation, characteristic of the vacuolar membrane (tonoplast). A portion (20%) of this intravesicular Ca²⁺ store is specifically released by inositol 1,4,5-trisphosphate (InsP₃) with a K _0.5 = 0.54 μM. InsP₃,-mediated Ca²⁺ release is eliminated by low concentrations of heparin (K _0.5 = 86 nM). This result highlights the conservation between the animal and plant inositol 1,4,5-trisphosphate-mediated Ca²⁺ release mechanisms, despite the fact that they are located at different intracellular membranes.

【 授权许可】

Unknown