The transmembrane topology of the α subunit from the ATPase in Escherichia coli analyzed by PhoA protein fusions

FEBS Letters

Michelsen, Ole¹  Bjørbæk, Christian¹  Foërsom, Vibeke¹ 

[1] Department of Microbiology, Technical University of Denmark, Building 221, DK 2800 Lyngby, Denmark

关键词: F0-ATPase;    Subunit α topology;    PhoA protein-fusion;    X-P;    5-bromo-4-chloro-3-indolylphosphate;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    F1;    cytoplasmic ATPase part of the H+ -ATPase complex;    F0;    membrane part of the ATPase complex;    PhoA;    alkaline phosphatase enzyme;    bla;    β-lactamase gene;    Km;    kanamycin;    Amp;    ampicillin;   

DOI  :  10.1016/0014-5793(90)80058-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The atpB gene encodes the α subunit of the H⁺-ATPase of E. coli. The topology of this membrane protein has been analyzed by PhoA fusions. The results support an eight transmembrane segment model that is consistent with the hydropathic profile.

【 授权许可】

Unknown