Small conductance chloride channels in the apical membrane of thyroid cells

FEBS Letters

Champigny, G.¹  Lazdunski, M.¹  Mauchamp, J.²  Gérard, C.²  Verrier, B.² 

[1] Institut de Pharmacologie Moléculaire et Cellulaire, UPR 411 CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 ValbonneFrance;Unité INSERM 270, Faculté de Médecine Secteur Nord, boulevard Pierre Dramard, 13326 Marseille Cédex 15, France

关键词: Chloride channel;    Thyroid cell;    Patch-clamp;   

DOI  :  10.1016/0014-5793(90)80024-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A small conductance chloride channel has been identified on the apical membrane of porcine thyroid cells using the patch-clamp technique. In cell attached membrane patches with NaCl in the pipette, the single channel conductance is 5.5 pS. The channel is highly selective for chloride over gluconate and iodide, and is impermeable to Na⁺, K⁺ and tetraethylammonium ions. The open state probability of the channel is not affected by voltage. The channel activity disappears after excision of the patch. The Cl⁻ channel Mocker 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB) did not affect the activity of the thyroid Cl⁻ channels. Treatment of thyroid cells with 8-(4-chlorophenylthio)adenosine-3',5'-cyclic monophosphate (8-chloro-cAMP) (0.5 mM) prior to giga-seal formation increased Cl⁻ channel activity in the apical membrane of thyroid cells.

【 授权许可】

Unknown