Lipoxins are major lipoxygenase products of rainbow trout macrophages

FEBS Letters

Pettitt, Trevor R.¹  Rowley, Andrew F.¹  Secombes, Chris J.² 

[1] Biochemical and Physiological Research Group, School of Biological Sciences, University College of Swansea, Singleton Park, Swansea, SA2 8PP, Wales UK;Deparfment of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN, Scotland

关键词: Macrophage;    Lipoxin;    Leukotriene;    Lipoxygenase product;    (Rainbow trout;    Saimo gairdneri);    Hete;    hydroxy-5;    8;    11;    13 eicosatetraenoate;    LXA4;    lipoxin A4;    LXA5;    lipoxin A5;    LXB4;    lipoxin B4;    LTB4 leukotriene B4;    LTB5;    leukotriene B5;    LTC4;    leukotriene C4;   

DOI  :  10.1016/0014-5793(89)81520-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rainbow trout macrophages synthesize lipoxins as major lipoxygenase products entirely from endogenous fatty acids. High-performance liquid Chromatographic analysis of the supernatants from macrophages challenged with calcium ionophore A23187 revealed a range of lipoxygenase products including mono-hydroxy fatty acids, leukotrienes B₄ and B₅ and four major peaks with retention times and UV spectra characteristic of lipoxins (λ_max 302 nm). Cochromatography with authentic standards, UV spectroscopy and radiolabelling with (¹⁴C]arachidonate and eicosapentaenoate allowed tentative identification of the two largest peaks as lipoxin A₄ and A₅.

【 授权许可】

Unknown