期刊论文详细信息
FEBS Letters | |
Surfactant enhancement of polyethyleneglycol‐induced cell fusion | |
Goñi, Félix M.1  Barberá-Guillem, Emilio2  Partearroyo, M.Aránzazu1  Mencía, Mario1  Prado, Adelina2  | |
[1] Department of Biochemistry, University of the Basque Country, PO Box 644, 48080 Bilbao, Spain;Department of Cell Biology University of the Basque Country, PO Box 644, 48080 Bilbao, Spain | |
关键词: Cell fusion; Polyethyleneglycol; Surfactant; (B16 melanoma); CHAPS; 3((3-cholamidopropyl) dimethylammonio)-1-propanesulphonate; CTAB; cetyltrimethylammonium bromide; PC; phosphatidylcholine; SDS; sodium dodecylsulphate; | |
DOI : 10.1016/0014-5793(89)81515-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
B 16 mouse melanoma cells in monolayers may be satisfactorily fused with 50% PEG 1500. However, pre-treatment with detergents in solution at low concentrations significantly increases PEG fusion, up to 8-fold in some instances, without impairing cell viability. The practical and mechanistical implications of this finding are discussed.
【 授权许可】
Unknown
【 预 览 】
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RO201912020292862ZK.pdf | 323KB | download |