Surfactant enhancement of polyethyleneglycol‐induced cell fusion

FEBS Letters

Goñi, Félix M.¹  Barberá-Guillem, Emilio²  Partearroyo, M.Aránzazu¹  Mencía, Mario¹  Prado, Adelina² 

[1] Department of Biochemistry, University of the Basque Country, PO Box 644, 48080 Bilbao, Spain;Department of Cell Biology University of the Basque Country, PO Box 644, 48080 Bilbao, Spain

关键词: Cell fusion;    Polyethyleneglycol;    Surfactant;    (B16 melanoma);    CHAPS;    3((3-cholamidopropyl) dimethylammonio)-1-propanesulphonate;    CTAB;    cetyltrimethylammonium bromide;    PC;    phosphatidylcholine;    SDS;    sodium dodecylsulphate;   

DOI  :  10.1016/0014-5793(89)81515-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

B 16 mouse melanoma cells in monolayers may be satisfactorily fused with 50% PEG 1500. However, pre-treatment with detergents in solution at low concentrations significantly increases PEG fusion, up to 8-fold in some instances, without impairing cell viability. The practical and mechanistical implications of this finding are discussed.

【 授权许可】

Unknown