Glucose‐induced oscillations of intracellular Ca2+ concentration resembling bursting electrical activity in single mouse islets of Langerhans

FEBS Letters

Soria, Bernat¹  Rosario, Luis M.¹  Santos, Rosa M.¹  Valdeolmillos, Miguel¹  Contreras, Diego¹ 

[1] Department of Physiology, School of Medicine, University of Alicante, Alicante, Spain

关键词: Islet of Langerhans;    Indo-1;    Ca2+ concentration;    intracellular;    Ca2+ oscillation;    Electrical activity;    Intercellular coupling;   

DOI  :  10.1016/0014-5793(89)81484-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intracellular Ca²⁺ levels were monitored in single, acutely isolated mouse islets of Langerhans by dual emission Indo-1 fluorometry. High-frequency (3.1 min⁻¹) [Ca²⁺]_i, oscillations with a brief rising time (1–2 s) and 10 s half-width (‘fast’ oscillations) were detected in 11 mM glucose. Raising the glucose concentration to 16.7 mM increased the duration of these oscillations, which were otherwise absent in 5.5 mM glucose. [Ca²⁺]_i waves of lower frequency (0.5 min⁻¹) and longer rising time (‘slow’ oscillations) were also recorded. The data indicate that “fast” oscillations are directly related to β-cell bursting electrical activity, and suggest the existence of extensive networks of electrically coupled cells in the islet.

【 授权许可】

Unknown