FEBS Letters | |
Glucose‐induced oscillations of intracellular Ca2+ concentration resembling bursting electrical activity in single mouse islets of Langerhans | |
Soria, Bernat1  Rosario, Luis M.1  Santos, Rosa M.1  Valdeolmillos, Miguel1  Contreras, Diego1  | |
[1] Department of Physiology, School of Medicine, University of Alicante, Alicante, Spain | |
关键词: Islet of Langerhans; Indo-1; Ca2+ concentration; intracellular; Ca2+ oscillation; Electrical activity; Intercellular coupling; | |
DOI : 10.1016/0014-5793(89)81484-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Intracellular Ca2+ levels were monitored in single, acutely isolated mouse islets of Langerhans by dual emission Indo-1 fluorometry. High-frequency (3.1 min−1) [Ca2+]i, oscillations with a brief rising time (1–2 s) and 10 s half-width (‘fast’ oscillations) were detected in 11 mM glucose. Raising the glucose concentration to 16.7 mM increased the duration of these oscillations, which were otherwise absent in 5.5 mM glucose. [Ca2+]i waves of lower frequency (0.5 min−1) and longer rising time (‘slow’ oscillations) were also recorded. The data indicate that “fast” oscillations are directly related to β-cell bursting electrical activity, and suggest the existence of extensive networks of electrically coupled cells in the islet.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020292830ZK.pdf | 526KB | download |