Evidence for reversible multiple redox transformations of [3Fe‐4S] clusters

FEBS Letters

Armstrong, Fraser A.²  George, Simon J.¹  Hatchikian, E.Claude³  Thomson, Andrew J.¹  Butt, Julea N.² 

[1] School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, England;Inorganic Chemistry Laboratory, South Parks Road, Oxford OX1 3QR England;Laboratoire de Chimie Bacterienne, C.N.R.S., PB 71, 13277 Marseille, France

关键词: Ferredoxin;    Iron-sulphur;    Electron transfer;    Voltammetry;    Electrochemistry;    (Desulfovibrio africanus);   

DOI  :  10.1016/0014-5793(89)81483-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Analysis of the diffusionless cyclic voltammetry of ferredoxin III from Desulfovibrio africanus, co-adsorbed with neomycin as an electroactive film on pyrolytic graphite ‘edge’ electrodes, shows that the reduced [3Fe-4S]⁰ cluster undergoes further fast, chemically reversible, two-electron reduction, at a potential E ⁰' = ca. −720 mV (pH 7.15, 0°C). The pH dependence of E ⁰' (pH 6.25–7.80) indicates net transfer of two H⁺. Observation of similar voltammetric waves in other proteins specifically containing [3Fe-4S] centres suggests that extensive redox activity and stabilisation of what is formally an all-Fe(II) species, may be a common, perhaps characteristic, feature of this cluster type.

【 授权许可】

Unknown