Colicin N and its thermolytic fragment induce phospholipid vesicle fusion

FEBS Letters

Massotte, D.¹  Pattus, F.¹ 

[1] European Molecular Biology Laboratory, Meyerhofstrasse 1, Postfach 10.2209, D-6900 Heidelberg, FRG

关键词: Colicin N;    Membrane fusion;    Fluorescence energy transfer;    Thermolytic fragment;    N-NBD-PE;    N-(7-nitro-1;    2;    3-benzoaxadiazol)-phosphatidylethanolamine;    N-Rho-PE;    N-(lissamine-rhodamine-sulfonyl)phosphatidylethanolamine;   

DOI  :  10.1016/0014-5793(89)81593-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Colicin N, a bacteriocin encoded on a plasmid belonging to the pore-forming class of colicins, induces phospholipid vesicle fusion at acidic pH as demonstrated by fluorescence resonance energy transfer. Its C-terminal thermolytic fragment has properties very similar to the native molecule. The fusion is protein concentration-dependent and is regulated by (a) group(s) with a pK of approximately 4.6. The physiological relevance of this characteristic common to all colicins tested so far is discussed.

【 授权许可】

Unknown