Parallel DNA: generation of a duplex between two Drosophila sequences in vitro

FEBS Letters

Tchurikov, Nickolai A.¹  Chernov, Boris K.³  Nechipurenko, Yurii D.²  Golova, Yulia B.³ 

[1] Department of Nucleic Acids Biosynthesis, Engelhardt Institute of Molecular Biology, the USSR Academy of Sciences, Vavilov str., 32,117984 Moscow B-334, USSR;Department of Biopolymers Physics, Engelhardt Institute of Molecular Biology, the USSR Academy of Sciences, Vavilov str., 32,117984 Moscow B-334, USSR;Department of Genetic Engineering, Engelhardt Institute of Molecular Biology, the USSR Academy of Sciences, Vavilov str., 32,117984 Moscow B-334, USSR

关键词: DNA;    parallel;    Suffix element;    Gene;    Adh;    Melting;    Hoechst 33 258;    CD;    (Drosophila);    Adh;    alcohol dehydrogenase gene of Drosophila melanogaster;    PC;    parallel construction containing two parallel DNA strands;    AC;    antiparallel construction containing two antiparallel DNA strands;    SS;    single-stranded DNA;    CD;    circular dichroism;   

DOI  :  10.1016/0014-5793(89)81585-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have observed the existence of a parallel complementary region between two Drosophila DNA sequences, fragments of the suffix [(1986) EMBO J, 5, 2341-2347] and a 5'-non-coding sequence of the alcohol dehydrogenase gene [(1983) Cell 33, 125-133]. The region includes ~40 bp, 76% of which are complementary in the same polarity. Synthetic complementary 16 bp oligonucleotides corresponding to this region which were bound by the 5'-ends through a 1.6-hexanediol bridge form a duplex which displays both melting and annealing as judged by UV absorbance. Anti parallel complementary 16 bp long oligonucleotides bound by the 5'-3' ends through the same bridge and a single-strand sequence were used as controls. The Hoechst 33 258 drug binds to this parallel duplex of DNA; however, the properties of such a complex testify against the B-form of the duplex.

【 授权许可】

Unknown