Amino acid sequences of cytotoxin‐like basic proteins derived from cobra venoms

FEBS Letters

Takechi, Masayuki²  Ikeda, Kiyoshi³  Inoue, Seiji³  Ohkura, Kazuto³  Tsujino, Michiyo¹  Okumura, Kohji¹  Hayashi, Kyozo⁴  Tanaka, Yasuo² 

[1] Faculty of Pharmacy, Kinki University, Higashiosaka 577 Japan;Department of Biochemistry, Osaka University of Pharmaceutical Sciences, Kawai, Matsubara, Osaka 580 Japan;Department of Pharmacology, Gifu Pharmaceutical University, Gifu 502, Japan

关键词: Amino acid sequence;    Cytotoxin;    Cardiotoxin;    Cytotoxin-like basic protein;    (Naja naja;    Naja naja atra);    CLBP;    cytotoxin-like basic protein;    LCBP;    less-cytotoxic basic polypeptide;    HPLC;    high-performance liquid chromatography;    Cm;    S-carboxymethyl;    Cmc;    S-carboxymethylcysteine;    N;    amino;    C;    carboxyl;   

DOI  :  10.1016/0014-5793(89)81561-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amino acid sequences of cytotoxin-like basic proteins (CLBPs), purified from the venoms of Formosan cobra (Naja naja atra) and Indian cobra (Naja naja), were reinvestigated. The determined sequences differed from those reported previously by Takechi et al. [(1985) Biochem. Int. 11, 795-802; (1987) Biochem. Int. 14, 145-152]. The sequence of CLBPs at residues 25-30 was found to be hydrophilic as compared with those of cytotoxins. The difference in the hydrophobicity of this region might be responsible for the difference in their cytotoxic activities.

【 授权许可】

Unknown