Characterization of two different peptides from the venom of the scorpion Buthus sindicus

FEBS Letters

Shafqat, Jawed¹  Beg, Obaid U.¹  Fazal, Annul¹  Zaidi, Zafar H.¹  Jörnvall, Hans¹ 

[1] HEJ Research Institute of Chemistry, University of Karachi, Karachi-32, Pakistan

关键词: Scorpion venom;    Half cystine;    Peptide characterization;    Amino acid sequence;    Homology;   

DOI  :  10.1016/0014-5793(89)81548-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two disulfide-rich, low-molecular mass peptides (~3 kDa and ~4 kDa) have been isolated from Buthus sindicus venom using ion-exchange and reverse-phase HPLC. Peptide I has 35 residues with 8 half-cystine residues and is clearly related to four-disulfide core proteins of the neurophysin type and to toxins of other scorpion species (55–63% residue identity). Peptide II, present in low yield, has 28 residues with 6 half-cystine residues and a structure largely dissimilar from that of peptide I and other characterized toxins, although probably still a member of the disulfide core peptide type. Consequently, scorpion venom contains, in addition to toxins characterized before, toxin-like compounds with distant relationships.

【 授权许可】

Unknown