Altered patterns of protein phosphorylation in articular chondrocytes treated with interleukin‐1 or synovial cytokines

FEBS Letters

Hulkower, K.I.¹  Evans, C.H.¹  Georgescu, H.I.¹ 

[1] Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery and Department of Microbiology, Biochemistry and Molecular Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA

关键词: Interleukin-1;    Synovial cytokine;    Chondrocyte-activating factor;    Protein phosphorylation;    Signal transduction;    Protein kinase;    IL-1;    interleukin-1;    PGE2;    prostaglandin E2;    CAP;    chondrocyte-activating factor;   

DOI  :  10.1016/0014-5793(89)81540-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cultures of lapine articular chondrocytes were exposed to purified, human, recombinant interleukin-1 α or partially purified preparations of lapine, synovial, cytokines in the presence of [³²P]orthophosphate. After 30 min incubation, phosphoproteins were extracted from the cells, separated by two-dimensional gel electrophoresis and visualized autoradiographically. Analysis of the autoradiograms revealed that interleukin-1 and the synovial factors produced marked changes in the pattern of protein phosphorylation. The synovial cytokines induced many of the same changes as interleukin-1, as well as a number of unique changes. This finding is consistent with the notion that, in addition to interleukin-1, synoviocytes secrete other cytokines which modulate the metabolism of chondrocytes. These data support the idea that signal transduction in chondrocytes responding to interleukin-1 involves the activation of one or more protein kinases.

【 授权许可】

Unknown