FEBS Letters | |
Altered patterns of protein phosphorylation in articular chondrocytes treated with interleukin‐1 or synovial cytokines | |
Hulkower, K.I.1  Evans, C.H.1  Georgescu, H.I.1  | |
[1] Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery and Department of Microbiology, Biochemistry and Molecular Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA | |
关键词: Interleukin-1; Synovial cytokine; Chondrocyte-activating factor; Protein phosphorylation; Signal transduction; Protein kinase; IL-1; interleukin-1; PGE2; prostaglandin E2; CAP; chondrocyte-activating factor; | |
DOI : 10.1016/0014-5793(89)81540-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Cultures of lapine articular chondrocytes were exposed to purified, human, recombinant interleukin-1 α or partially purified preparations of lapine, synovial, cytokines in the presence of [32P]orthophosphate. After 30 min incubation, phosphoproteins were extracted from the cells, separated by two-dimensional gel electrophoresis and visualized autoradiographically. Analysis of the autoradiograms revealed that interleukin-1 and the synovial factors produced marked changes in the pattern of protein phosphorylation. The synovial cytokines induced many of the same changes as interleukin-1, as well as a number of unique changes. This finding is consistent with the notion that, in addition to interleukin-1, synoviocytes secrete other cytokines which modulate the metabolism of chondrocytes. These data support the idea that signal transduction in chondrocytes responding to interleukin-1 involves the activation of one or more protein kinases.
【 授权许可】
Unknown
【 预 览 】
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RO201912020292715ZK.pdf | 458KB | download |