γ‐Thrombin‐induced phospholipase A2 activation in rabbit platelets: Comparison with α‐thrombin

FEBS Letters

Jandrot-Perrus, Martine²  Touqui, Lhousseine¹  Vargaftig, B.Boris¹ 

[1] Unité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur/INSERM No 285, 25 rue Dr. Roux, 75015 Paris, France;Laboratoire d'Hémostase et de Thrombose, Faculté Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France

关键词: Thrombin;    γ-;    Phospholipase A2;    Platelet;    AA;    arachidonic acid;    PAF;    platelet-activating factor;    PKC;    protein kinase C;    PLA2;    phospholipase A2;    EC50;    effective concentration giving 50% of maximal response;    NPGB;    p−nitrophenyl-p-guanidino benzoate;   

DOI  :  10.1016/0014-5793(89)81142-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

γ-Thrombin stimulated release of [³H]arachidonic acid ([³H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA₂ activation, were observed after a prolonged lag and to a lower extent when compared to those induced by α-thrombin which evoked a much higher elevation in intracellular calcium. This elevation together with [³H]AA release were markedly reduced by EDTA. However, addition of ionophore A23187 enhanced the release of [³H]AA by γ-thrombin to the levels similar to those of α-thrombin. We conclude that γ-thrombin is able to activate PLA₂ and suggest that calcium influx may be a limiting factor for this activation.

【 授权许可】

Unknown