Thyroid hormone effect on rat heart mitochondrial proteins and affinity labeling with N‐bromoacetyl‐3,3′,5‐triiodo‐L‐thyronine Lack of direct effect on the adenine nucleotide translocase

FEBS Letters

Hesch, Rolf-Dieter²  Köhrle, Josef²  Rokos, Hartmut¹  Rasmussen, Ulla B.² 

[1] Forschungslabor, Henning Berlin GmbH, D-1000 Berlin 42, Germany;Abteilung Endokrinologie, Medizinische Hochschule Hannover, D-3000 Hannover 61, FRG

关键词: Bromoacetyl-3;    3′;    5-tri[3′-125I]iodo-L-thyronine;    N-;    Adenine nucleotide translocase;    Thyroid hormone;    (Rat heart mitochondria);   

DOI  :  10.1016/0014-5793(89)81128-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-bromoacetyl-3,3′,5-tri[3′-¹²⁵I]iodo-L-thyronine was used to label intact heart mitochondria from eu-, hypo- and hyperthyroid rats in order to identify proteins involved in T3-regulated mitochondrial processes. The results show strong labeling, competed for by T3 and other analogues, of two proteins with a molecular mass of 48 000 and 49 200 Da. No labeling is seen of the adenine nucleotide translocase, a likely target, neither at 0°C, at room temperature, nor after preincubation with the substrates or specific inhibitors. No difference in labeling intensity or distribution is seen in mitochondria from eu-, hypo- or hyperthyroid rats, and the abundance of the adenine nucleotide translocase is unchanged, but five other proteins show differential abundance.

【 授权许可】

Unknown