DNA fingerprints revealing common and divergent human DNA methylation patterns

FEBS Letters

Ramael, Marc¹  Raeymaekers, Peter²  Berneman, Zwi N.¹  Vanhoof, Greet²  Vandenberghe, Antoon²  Van Broeckhoven, Christine²  Peetermans, Marc E.¹  Van Bockstaele, Dirk¹ 

[1]Laboratory of Experimental Hematology Department of Biochemistry, University of Antwerp (UIA), Belgium

[2]Laboratory of Medical Biotechnology, Department of Biochemistry, University of Antwerp (UIA), Belgium

关键词: DNA methylation;    DNA fingerprinting;    Development;   

DOI  :  10.1016/0014-5793(89)81096-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We compared DNA fingerprints of different cell populations from the same individuals, after separate digestion with the isoschizomers MboI and Sau3A. Methylation differences were observed within every individual when comparing fingerprints of Sau3A- with MboI-digested DNA, and of Sau3A-digested sperm with somatic DNA. In some cases, differences were also detected between fingerprints of Sau3A-digested somatic DNA originating from various cell sources. Methylation patterns common to all cell populations examined, including the germline, were observed with a higher frequency than divergent ones. These ‘common methylations’ are most likely to find their origin during early embryogenesis.

【 授权许可】

Unknown