RNA degrading activity is tightly associated with the multicatalytic proteinase, ingensin

FEBS Letters

Sugita, Hideo¹  Tsukahara, Toshifumi¹  Ishiura, Shoichi¹  Ogawa, Toshio¹  Tanaka, Ken-ichi¹  Funabiki, Ryuhei² 

[1] National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187 Japan;Department of Agricultural Chemistry, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183, Japan

关键词: Proteinase;    RNA;    Ingensin;    RNA-degradation;   

DOI  :  10.1016/0014-5793(89)81086-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The multicatalytic proteinase, ingensin, was purified to homogeneity from chicken liver. rRNA-degrading activity was co-eluted with the purified multicatalytic proteinase from a TSK-3000SW column. This RNA-degrading activity was inactivated by heat treatment and the addition of a low concentration of SDS. Therefore, the RNA-degrading activity co-eluted with the multicatalytic proteinase was not due to contamination by low-molecular-mass RNases. These results strongly suggest that this RNA-degrading activity was tightly associated with the multicatalytic proteinase, ingensin.

【 授权许可】

Unknown